Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.
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ABSTRACT:
SUBMITTER: Hallez R
PROVIDER: S-EPMC1828643 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
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Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.
Hallez Régis R Letesson Jean-Jacques JJ Vandenhaute Jean J De Bolle Xavier X
Applied and environmental microbiology 20061215 4
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus. ...[more]
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