Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
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ABSTRACT:
SUBMITTER: Louis P
PROVIDER: S-EPMC1828812 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
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Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
Louis Petra P Flint Harry J HJ
Applied and environmental microbiology 20070126 6
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota. ...[more]
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