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Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains.


ABSTRACT: We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.

SUBMITTER: Ito K 

PROVIDER: S-EPMC1829111 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature

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Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains.

Ito Kenitiro K   Iida Mariko M   Yamazaki Mitsugu M   Moriya Kazuo K   Moroishi Sanae S   Yatsuyanagi Jun J   Kurazono Takayuki T   Hiruta Noriaki N   Ratchtrachenchai Orn-Anong OA  

Journal of clinical microbiology 20070117 3


We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes. ...[more]

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