A mechanism for cell-cycle regulation of MAP kinase signaling in a yeast differentiation pathway.
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ABSTRACT:
SUBMITTER: Strickfaden SC
PROVIDER: S-EPMC1847584 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Publications
A mechanism for cell-cycle regulation of MAP kinase signaling in a yeast differentiation pathway.
Strickfaden Shelly C SC Winters Matthew J MJ Ben-Ari Giora G Lamson Rachel E RE Tyers Mike M Pryciak Peter M PM
Cell 20070201 3
Yeast cells arrest in the G1 phase of the cell cycle upon exposure to mating pheromones. As cells commit to a new cycle, G1 CDK activity (Cln/CDK) inhibits signaling through the mating MAPK cascade. Here we show that the target of this inhibition is Ste5, the MAPK cascade scaffold protein. Cln/CDK disrupts Ste5 membrane localization by phosphorylating a cluster of sites that flank a small, basic, membrane-binding motif in Ste5. Effective inhibition of Ste5 signaling requires multiple phosphoryla ...[more]
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