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Structural basis for the selection of glycosylated substrates by SCF(Fbs1) ubiquitin ligase.


ABSTRACT: The ubiquitin ligase complex SCF(Fbs1), which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway. In SCF ubiquitin ligases, a diverse array of F-box proteins confers substrate specificity. Fbs1/Fbx2, a member of the F-box protein family, recognizes high-mannose oligosaccharides. To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex. The mechanistic model indicated by the structures appears to be well conserved among the SCF ubiquitin ligases. The structure of the SBD-glycoprotein complex indicates that the SBD primarily recognizes Man(3)GlcNAc(2), thereby explaining the broad activity of the enzyme against various glycoproteins. Comparison of two crystal structures of the Skp1-Fbs1 complex revealed the relative motion of a linker segment between the F-box and the SBD domains, which might underlie the ability of the complex to recognize different acceptor lysine residues for ubiquitination.

SUBMITTER: Mizushima T 

PROVIDER: S-EPMC1851568 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Structural basis for the selection of glycosylated substrates by SCF(Fbs1) ubiquitin ligase.

Mizushima Tsunehiro T   Yoshida Yukiko Y   Kumanomidou Taichi T   Hasegawa Yuko Y   Suzuki Atsuo A   Yamane Takashi T   Tanaka Keiji K  

Proceedings of the National Academy of Sciences of the United States of America 20070326 14


The ubiquitin ligase complex SCF(Fbs1), which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway. In SCF ubiquitin ligases, a diverse array of F-box proteins confers substrate specificity. Fbs1/Fbx2, a member of the F-box protein family, recognizes high-mannose oligosaccharides. To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain  ...[more]

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