Unknown

Dataset Information

0

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes.


ABSTRACT: In all three domains of life, DNA replication begins at specialized loci termed replication origins. In bacteria, replication initiates from a single, clearly defined site. In contrast, eukaryotic organisms exploit a multitude of replication origins, dividing their genomes into an array of short contiguous units. Recently, the multiple replication origin paradigm has also been demonstrated within the archaeal domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has three replication origins. However, the evolutionary mechanism driving the progression from single to multiple origin usage remains unclear. Here, we demonstrate that Aeropyrum pernix, a distant relative of Sulfolobus, has two origins. Comparison with the Sulfolobus origins provides evidence for evolution of replicon complexity by capture of extrachromosomal genetic elements. We additionally identify a previously unrecognized candidate archaeal initiator protein that is distantly related to eukaryotic Cdt1. Our data thus provide evidence that horizontal gene transfer, in addition to its well-established role in contributing to the information content of chromosomes, may fundamentally alter the manner in which the host chromosome is replicated.

SUBMITTER: Robinson NP 

PROVIDER: S-EPMC1851573 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes.

Robinson Nicholas P NP   Bell Stephen D SD  

Proceedings of the National Academy of Sciences of the United States of America 20070328 14


In all three domains of life, DNA replication begins at specialized loci termed replication origins. In bacteria, replication initiates from a single, clearly defined site. In contrast, eukaryotic organisms exploit a multitude of replication origins, dividing their genomes into an array of short contiguous units. Recently, the multiple replication origin paradigm has also been demonstrated within the archaeal domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has t  ...[more]

Similar Datasets

| S-EPMC4402960 | biostudies-literature
| S-EPMC262328 | biostudies-other
| S-EPMC7157519 | biostudies-literature
| S-EPMC3528665 | biostudies-literature
| S-EPMC4724246 | biostudies-literature
| S-EPMC332231 | biostudies-other
| S-EPMC4165761 | biostudies-literature
2020-12-28 | GSE159537 | GEO
| S-EPMC4164010 | biostudies-literature
| S-EPMC4441112 | biostudies-literature