Dataset Information

CCR4/NOT complex associates with the proteasome and regulates histone methylation.

ABSTRACT: The proteasome regulates histone lysine methylation and gene transcription, but how it does so is poorly understood. To better understand this process, we used the epistatic miniarray profile (E-MAP) approach to identify factors that genetically interact with proteasomal subunits. In addition to members of the Set1 complex that mediate histone H3 lysine 4 methylation (H3K4me), we found that deleting members of the CCR4/NOT mRNA processing complex exhibit synthetic phenotypes when combined with proteasome mutants. Further biochemical analyses revealed physical associations between CCR4/NOT and the proteasome in vivo. Consistent with the genetic and biochemical interactions linking CCR4/NOT with proteasome and Set1-mediated methylation, we find that loss of Not4 decreases global and gene-specific H3K4 trimethylation (H3K4me3) and decreases 19S proteasome recruitment to the PMA1 gene. Similar to proteasome regulation of histone methylation, loss of CCR4/NOT members does not affect ubiquitinated H2B. Mapping of Not4 identified the RING finger domain as essential for H3K4me3, suggesting a role for ubiquitin in this process. Consistent with this idea, loss of the Not4-interacting protein Ubc4, a known ubiquitin-conjugating enzyme, decreases H3K4me3. These studies implicate CCR4/NOT in the regulation of H3K4me3 through a ubiquitin-dependent pathway that likely involves the proteasome.

SUBMITTER: Laribee RN

PROVIDER: S-EPMC1851578 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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CCR4/NOT complex associates with the proteasome and regulates histone methylation.

Laribee R Nicholas RN Shibata Yoichiro Y Mersman Douglas P DP Collins Sean R SR Kemmeren Patrick P Roguev Assen A Weissman Jonathan S JS Briggs Scott D SD Krogan Nevan J NJ Strahl Brian D BD

Proceedings of the National Academy of Sciences of the United States of America 20070326 14

The proteasome regulates histone lysine methylation and gene transcription, but how it does so is poorly understood. To better understand this process, we used the epistatic miniarray profile (E-MAP) approach to identify factors that genetically interact with proteasomal subunits. In addition to members of the Set1 complex that mediate histone H3 lysine 4 methylation (H3K4me), we found that deleting members of the CCR4/NOT mRNA processing complex exhibit synthetic phenotypes when combined with p ...[more]

PMID: 17389396

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Dataset Information

CCR4/NOT complex associates with the proteasome and regulates histone methylation.

Publications

CCR4/NOT complex associates with the proteasome and regulates histone methylation.

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