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QBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.

ABSTRACT: Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.

SUBMITTER: Hellemans J

PROVIDER: S-EPMC1852402 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature

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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.

Hellemans Jan J Mortier Geert G De Paepe Anne A Speleman Frank F Vandesompele Jo J

Genome biology 20070101 2

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data ...[more]

PMID: 17291332

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