Ontology highlight
ABSTRACT:
SUBMITTER: Frantom PA
PROVIDER: S-EPMC1855043 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Frantom Patrick A PA Fitzpatrick Paul F PF
Journal of the American Chemical Society 20031201 52
Tyrosine hydroxylase (TyrH) catalyzes the hydroxylation of tyrosine to dihydroxyphenylalanine. In the proposed mechanism, a ferryl-oxo species attacks the aromatic ring of tyrosine, forming a cationic intermediate. However, no significant isotope effect is found for wild-type TyrH when 3,5-2H2-tyrosine is used as a substrate. The isotope effect has now been determined with 3,5-2H2-tyrosine using mutant forms of TyrH in which the oxidation of the pterin is uncoupled from hydroxylation of the amin ...[more]