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Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains.


ABSTRACT: Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.

SUBMITTER: Bidet P 

PROVIDER: S-EPMC1855671 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains.

Bidet Philippe P   Metais Arnaud A   Mahjoub-Messai Farah F   Durand Lionel L   Dehem Marie M   Aujard Yannick Y   Bingen Edouard E   Nassif Xavier X   Bonacorsi Stéphane S  

Applied and environmental microbiology 20070209 7


Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches. ...[more]

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