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Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125.

ABSTRACT: Retinoic acid-inducible gene I (RIG-I) plays a pivotal role in the regulation of cytokine production induced by pathogens. The RIG-I also augments the production of IFN and other cytokines via an amplification circuit. Because the production of cytokines is closely controlled, up- and down-regulation of RIG-I signaling also needs strict regulation. The mechanism of this regulation, however, remains elusive. Here, we found that RIG-I undergoes proteasomal degradation after conjugation to ubiquitin by RNF125. Further, RNF125 conjugates ubiquitin to MDA5, a family protein of RIG-I as well as IPS-1, which is also a downstream protein of RIG-I signaling that results in suppressing the functions of these proteins. Because RNF125 is enhanced by IFN, these functions constitute a negative regulatory loop circuit for IFN production.

SUBMITTER: Arimoto K

PROVIDER: S-EPMC1863485 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125.

Arimoto Kei-ichiro K Takahashi Hitoshi H Hishiki Takayuki T Konishi Hideyuki H Fujita Takashi T Shimotohno Kunitada K

Proceedings of the National Academy of Sciences of the United States of America 20070425 18

Retinoic acid-inducible gene I (RIG-I) plays a pivotal role in the regulation of cytokine production induced by pathogens. The RIG-I also augments the production of IFN and other cytokines via an amplification circuit. Because the production of cytokines is closely controlled, up- and down-regulation of RIG-I signaling also needs strict regulation. The mechanism of this regulation, however, remains elusive. Here, we found that RIG-I undergoes proteasomal degradation after conjugation to ubiquiti ...[more]

PMID: 17460044

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