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A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals.

ABSTRACT: Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic DeltaguaB mutant, a nonflagellated DeltafliC mutant, and an auxotrophic and nonflagellated DeltaguaB DeltafliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The DeltafliC mutant was pathogenic in orally infected BALB/c mice, while the DeltaguaB mutant was attenuated and conferred protection against a challenge with the pathogenic parent strain. The DeltaguaB DeltafliC double mutant was totally asymptomatic and conferred better protection than the DeltaguaB mutant. This indicates that the major flagellar protein flagellin is not required for efficient vaccination of BALB/c mice against Salmonella infection. The DeltaguaB DeltafliC mutant was also safe for vaccination of 1-day-old chickens. After two immunizations, it induced statistically significant protection against infection of the internal organs of the birds by a virulent S. enterica serovar Enteritidis challenge strain but not against intestinal colonization. These data demonstrate that nonflagellated attenuated Salmonella mutants can be used as marker vaccines.

SUBMITTER: Adriaensen C

PROVIDER: S-EPMC1865732 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals.

Adriaensen Connie C De Greve Henri H Tian Jean Q JQ De Craeye Stéphane S Gubbels Eline E Eeckhaut Venessa V Van Immerseel Filip F Ducatelle Richard R Kumar Mahesh M Hernalsteens Jean-Pierre JP

Infection and immunity 20070129 5

Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic DeltaguaB mutant, a nonflagellated DeltafliC mutant, and an auxotrophic and nonflagellated DeltaguaB DeltafliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The DeltafliC mutant was patho ...[more]

PMID: 17261603

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Project description:Salmonella enterica serovar Enteritidis has emerged as a significant foodborne pathogen throughout the world and is commonly characterized by phage typing. In Canada phage types (PT) 4, 8 and 13 predominate and in 2005 a large foodborne PT13 outbreak occurred in the province of Ontario. The ability to link strains during this outbreak was difficult due to the apparent clonality of PT13 isolates in Canada, as there was a single dominant pulsed-field gel electrophoresis (PFGE) profile amongst epidemiologically linked human and food isolates as well as concurrent sporadic strains. The aim of this study was to perform comparative genomic hybridization (CGH), DNA sequence-based typing (SBT) genomic analyses, plasmid analyses, and automated repetitive sequence-based PCR (rep-PCR) to identify epidemiologically significant traits capable of subtyping S. Enteritidis PT13.CGH using an oligonucleotide array based upon chromosomal coding sequences of S. enterica serovar Typhimurium strain LT2 and the Salmonella genomic island 1 successfully determined major genetic differences between S. Typhimurium and S. Enteritidis PT13, but no significant strain-to-strain differences were observed between S. Enteritidis PT13 isolates. Individual loci (safA and fliC) that were identified as potentially divergent in the CGH data set were sequenced in a panel of S. Enteritidis strains, and no differences were detected between the PT13 strains. Additional sequence-based typing was performed at the fimA, mdh, manB, cyaA, citT, caiC, dmsA, ratA and STM0660 loci. Similarly, no diversity was observed amongst PT13 strains. Variation in plasmid content between PT13 strains was observed, but macrorestriction with BglII did not identify further differences. Automated rep-PCR patterns were variable between serovars, but S. Enteritidis PT13 strains could not be differentiated.None of the methods identified any significant variation between PT13 strains. Greater than 11,300 base pairs of sequence for each of seven S. Enteritidis PT13 strains were analyzed without detecting a single polymorphic site, although diversity between different phage types of S. Enteritidis was observed. These data suggest that Canadian S. Enteritidis PT13 strains are highly related genetically.

Dataset Information

A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals.

Publications

A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals.

Similar Datasets

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