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Colorimetric phage-based assay for detection of rifampin-resistant Mycobacterium tuberculosis.

ABSTRACT: Tests based on bacteriophage replication enable rapid screening of Mycobacterium tuberculosis for drug resistance. We describe a novel broth-based colorimetric method for detecting phage replication. When clinical isolates were tested by this novel method, high concordance was observed with both the traditional phage assay and gene mutation analysis for detection of resistance to rifampin.

SUBMITTER: McNerney R

PROVIDER: S-EPMC1865820 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Colorimetric phage-based assay for detection of rifampin-resistant Mycobacterium tuberculosis.

McNerney Ruth R Mallard Kim K Urassa Honorathy M R HM Lemma Eshetu E Donoghue Helen D HD

Journal of clinical microbiology 20070214 4

Tests based on bacteriophage replication enable rapid screening of Mycobacterium tuberculosis for drug resistance. We describe a novel broth-based colorimetric method for detecting phage replication. When clinical isolates were tested by this novel method, high concordance was observed with both the traditional phage assay and gene mutation analysis for detection of resistance to rifampin. ...[more]

PMID: 17301279

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Correction: Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis.

Project description:[This corrects the article DOI: 10.1371/journal.pone.0143444.].

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Discordance between Xpert MTB/RIF assay and Bactec MGIT 960 Culture System for detection of rifampin-resistant Mycobacterium tuberculosis isolates in a country with a low tuberculosis (TB) incidence.

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