Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
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ABSTRACT:
SUBMITTER: Di Costanzo L
PROVIDER: S-EPMC1866264 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Publications
Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
Di Costanzo Luigi L Gomez German A GA Christianson David W DW
Journal of molecular biology 20061110 2
Aldehyde dehydrogenases catalyze the oxidation of aldehyde substrates to the corresponding carboxylic acids. Lactaldehyde dehydrogenase from Escherichia coli (aldA gene product, P25553) is an NAD(+)-dependent enzyme implicated in the metabolism of l-fucose and l-rhamnose. During the heterologous expression and purification of taxadiene synthase from the Pacific yew, lactaldehyde dehydrogenase from E. coli was identified as a minor (</=5%) side-product subsequent to its unexpected crystallization ...[more]
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