Ontology highlight
ABSTRACT:
SUBMITTER: Brown DT
PROVIDER: S-EPMC1868459 | biostudies-literature | 2006 Mar
REPOSITORIES: biostudies-literature
Brown David T DT Izard Tina T Misteli Tom T
Nature structural & molecular biology 20060205 3
H1 linker histones stabilize the nucleosome, limit nucleosome mobility and facilitate the condensation of metazoan chromatin. Here, we have combined systematic mutagenesis, measurement of in vivo binding by photobleaching microscopy, and structural modeling to determine the binding geometry of the globular domain of the H1(0) linker histone variant within the nucleosome in unperturbed, native chromatin in vivo. We demonstrate the existence of two distinct DNA-binding sites within the globular do ...[more]