Normalization of two-channel microarrays accounting for experimental design and intensity-dependent relationships.
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ABSTRACT:
SUBMITTER: Dabney AR
PROVIDER: S-EPMC1868928 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Publications
Normalization of two-channel microarrays accounting for experimental design and intensity-dependent relationships.
Dabney Alan R AR Storey John D JD
Genome biology 20070101 3
In normalizing two-channel expression arrays, the ANOVA approach explicitly incorporates the experimental design in its model, and the MA plot-based approach accounts for intensity-dependent biases. However, both approaches can lead to inaccurate normalization in fairly common scenarios. We propose a method called efficient Common Array Dye Swap (eCADS) for normalizing two-channel microarrays that accounts for both experimental design and intensity-dependent biases. Under reasonable experimental ...[more]
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