A requirement for replication in activation of the ATR-dependent DNA damage checkpoint.
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ABSTRACT:
SUBMITTER: Lupardus PJ
PROVIDER: S-EPMC187437 | biostudies-literature | 2002 Sep
REPOSITORIES: biostudies-literature
Publications
A requirement for replication in activation of the ATR-dependent DNA damage checkpoint.
Lupardus Patrick J PJ Byun Tony T Yee Muh-Ching MC Hekmat-Nejad Mohammad M Cimprich Karlene A KA
Genes & development 20020901 18
Using the Xenopus egg extract system, we investigated the involvement of DNA replication in activation of the DNA damage checkpoint. We show here that DNA damage slows replication in a checkpoint-independent manner and is accompanied by replication-dependent recruitment of ATR and Rad1 to chromatin. We also find that the replication proteins RPA and Polalpha accumulate on chromatin following DNA damage. Finally, damage-induced Chk1 phosphorylation and checkpoint arrest are abrogated when replica ...[more]
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