Glycoprotein structural genomics: solving the glycosylation problem.
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ABSTRACT:
SUBMITTER: Chang VT
PROVIDER: S-EPMC1885966 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Publications
Glycoprotein structural genomics: solving the glycosylation problem.
Chang Veronica T VT Crispin Max M Aricescu A Radu AR Harvey David J DJ Nettleship Joanne E JE Fennelly Janet A JA Yu Chao C Boles Kent S KS Evans Edward J EJ Stuart David I DI Dwek Raymond A RA Jones E Yvonne EY Owens Raymond J RJ Davis Simon J SJ
Structure (London, England : 1993) 20070301 3
Glycoproteins present special problems for structural genomic analysis because they often require glycosylation in order to fold correctly, whereas their chemical and conformational heterogeneity generally inhibits crystallization. We show that the "glycosylation problem" can be solved by expressing glycoproteins transiently in mammalian cells in the presence of the N-glycosylation processing inhibitors, kifunensine or swainsonine. This allows the correct folding of the glycoproteins, but leaves ...[more]
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