Ontology highlight
ABSTRACT:
SUBMITTER: Chi A
PROVIDER: S-EPMC1892997 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Chi An A Huttenhower Curtis C Geer Lewis Y LY Coon Joshua J JJ Syka John E P JE Bai Dina L DL Shabanowitz Jeffrey J Burke Daniel J DJ Troyanskaya Olga G OG Hunt Donald F DF
Proceedings of the National Academy of Sciences of the United States of America 20070207 7
We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment for phosphopeptide fractionation and detection, gas phase ion/ion chemistry, electron transfer dissociation for peptide fragmentation, and the Open Mass Spectrometry Search Algorithm for phosphoprotein identification and assignment of phosphorylat ...[more]