Interrelationships between colonies, biofilms, and planktonic cells of Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa is a gram-negative bacterium and an opportunistic human pathogen that causes chronic infections in immunocompromised individuals. These infections are hard to treat, partly due to the high intrinsic resistance of the bacterium to clinically used antibiotics and partly due to the formation of antibiotic-tolerant biofilms. The three most common ways of growing bacteria in vitro are as planktonic cultures, colonies on agar plates, and biofilms in continuous-flow systems. Biofilms are known to express genes different from those of planktonic cells, and biofilm cells are generally believed to closely resemble planktonic cells in stationary phase. However, few, if any, studies have examined global gene expression in colonies. We used a proteomic approach to investigate the

SUBMITTER: Mikkelsen H 

PROVIDER: S-EPMC1899361 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature