Random screening for dominant-negative mutants of the cytomegalovirus nuclear egress protein M50.
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ABSTRACT:
SUBMITTER: Rupp B
PROVIDER: S-EPMC1900260 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
Publications
Random screening for dominant-negative mutants of the cytomegalovirus nuclear egress protein M50.
Rupp Brigitte B Ruzsics Zsolt Z Buser Christopher C Adler Barbara B Walther Paul P Koszinowski Ulrich H UH
Journal of virology 20070321 11
Inactivation of gene products by dominant-negative (DN) mutants is a powerful tool to assign functions to proteins. Here, we present a two-step procedure to establish a random screen for DN alleles, using the essential murine cytomegalovirus gene M50 as an example. First, loss-of-function mutants from a linker-scanning library were tested for inhibition of virus reconstitution with the help of FLP-mediated ectopic insertion of the mutants into the viral genome. Second, DN candidates were confirm ...[more]
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