Targeted access to the genomes of low-abundance organisms in complex microbial communities.

ABSTRACT: Current metagenomic approaches to the study of complex microbial consortia provide a glimpse into the community metabolism and occasionally allow genomic assemblies for the most abundant organisms. However, little information is gained for the members of the community present at low frequencies, especially those representing yet-uncultured taxa, which include the bulk of the diversity present in most environments. Here we used phylogenetically directed cell separation by fluorescence in situ hybridization and flow cytometry, followed by amplification and sequencing of a fraction of the genomic DNA of several bacterial cells that belong to the TM7 phylum. Partial genomic assembly allowed, for the first time, a look into the evolution and potential metabolism of a soil representative from th

SUBMITTER: Podar M 

PROVIDER: S-EPMC1907129 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature