Comparative analysis of bacterial genomes: identification of divergent regions in mycobacterial strains using an anchor-based approach.

ABSTRACT: Comparative genomic approaches are useful in identifying molecular differences between organisms. Currently available methods fail to identify small changes in genomes, such as expansion of short repetitive motifs and to analyse divergent sequences. In this report, we describe an anchor-based whole genome comparison (ABWGC) method. ABWGC is based on random sampling of anchor sequences from one genome, followed by analysis of sampled and homologous regions from the target genome. The method was applied to compare two strains of Mycobacterium tuberculosis CDC1551 and H37Rv. ABWGC was able to identify a total of 104 indels including 20 expansion of short repetitive sequences and five recombination events. It included 18 new unidentified genomic differences. ABWGC also identified 188 SNPs incl

SUBMITTER: Vishnoi A 

PROVIDER: S-EPMC1931498 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature