Ontology highlight
ABSTRACT:
SUBMITTER: Trefzer A
PROVIDER: S-EPMC1932781 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20070504 13
Discovery of the CYP107Z subfamily of cytochrome P450 oxidases (CYPs) led to an alternative biocatalytic synthesis of 4''-oxo-avermectin, a key intermediate for the commercial production of the semisynthetic insecticide emamectin. However, under industrial process conditions, these wild-type CYPs showed lower yields due to side product formation. Molecular evolution employing GeneReassembly was used to improve the regiospecificity of these enzymes by a combination of random mutagenesis, protein ...[more]