Ontology highlight
ABSTRACT:
SUBMITTER: Wang C
PROVIDER: S-EPMC1933516 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Wang Chunlin C Mitsuya Yumi Y Gharizadeh Baback B Ronaghi Mostafa M Shafer Robert W RW
Genome research 20070628 8
The detection of mutant spectra within a population of microorganisms is critical for the management of drug-resistant infections. We performed ultra-deep pyrosequencing to detect minor sequence variants in HIV-1 protease and reverse transcriptase (RT) genes from clinical plasma samples. We estimated empirical error rates from four HIV-1 plasmid clones and used them to develop a statistical approach to distinguish authentic minor variants from sequencing errors in eight clinical samples. Ultra-d ...[more]