Structure-function studies of the G-domain from human gem, a novel small G-protein.
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ABSTRACT:
SUBMITTER: Opatowsky Y
PROVIDER: S-EPMC1934412 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Publications
Structure-function studies of the G-domain from human gem, a novel small G-protein.
Opatowsky Yarden Y Sasson Yehezkel Y Shaked Isabella I Ward Yvona Y Chomsky-Hecht Orna O Litvak Yael Y Selinger Zvi Z Kelly Kathleen K Hirsch Joel A JA
FEBS letters 20061006 25
Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 A resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatial ...[more]
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