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Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma.

ABSTRACT: Cells undergoing apoptosis are characterized by decreased cell size due to changes in intracellular ion concentration and rapid, aquaporin (AQP)-dependent water movement out of the cell, events required for the activation of pro-apoptotic enzymes. The current study demonstrates AQP 8 and 9 expression is significantly decreased in hepatocellular carcinoma (HCC) versus normal liver. Isolation of hepatic tumor cells (H4IIE) and hepatocytes confirmed a lack of water movement across the H4IIE cell membrane via AQPs and identified an inherent resistance of H4IIE cells to apoptotic stimuli. In contrast, hepatocytes rapidly responded to osmotic challenge through AQP-dependent water movement and underwent cell death following apoptotic stimulation.

SUBMITTER: Jablonski EM

PROVIDER: S-EPMC1934939 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma.

Jablonski Elizabeth M EM Mattocks M Adrian MA Sokolov Eugene E Koniaris Leonidas G LG Hughes Francis M FM Fausto Nelson N Pierce Robert H RH McKillop Iain H IH

Cancer letters 20061103 1

Cells undergoing apoptosis are characterized by decreased cell size due to changes in intracellular ion concentration and rapid, aquaporin (AQP)-dependent water movement out of the cell, events required for the activation of pro-apoptotic enzymes. The current study demonstrates AQP 8 and 9 expression is significantly decreased in hepatocellular carcinoma (HCC) versus normal liver. Isolation of hepatic tumor cells (H4IIE) and hepatocytes confirmed a lack of water movement across the H4IIE cell me ...[more]

PMID: 17084522

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