Ontology highlight
ABSTRACT:
SUBMITTER: Luo G
PROVIDER: S-EPMC1937514 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Luo Guobin G Wang Mina M Konigsberg William H WH Xie X Sunney XS
Proceedings of the National Academy of Sciences of the United States of America 20070718 31
We report fluorescence assays for a functionally important conformational change in bacteriophage T7 DNA polymerase (T7 pol) that use the environmental sensitivity of a Cy3 dye attached to a DNA substrate. An increase in fluorescence intensity of Cy3 is observed at the single-molecule level, reflecting a conformational change within the T7 pol ternary complex upon binding of a dNTP substrate. This fluorescence change is believed to reflect the closing of the T7 pol fingers domain, which is cruci ...[more]