Dataset Information

Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs.

ABSTRACT: OBJECTIVE: Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS). METHODS: The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion. RESULTS: The presence of an erythromycin ribosome methylation gene (ermB or ermTR variant A) predicted in vitro constitutive or inducible resistance to clindamycin with a sensitivity of 93% (95% CI 86%-97%), specificity of 90% (95% CI 85%-93%), positive predictive value of 76% (95% CI 67%-84%), and negative predictive value of 97% (95% CI 94%-99%). CONCLUSION: This rapid and simple assay can predict in vitro susceptibility to clindamycin within two hours of isolation as opposed to 18-24 hours via disk diffusion. The assay might also be used to screen large numbers of batched isolates to establish the prevalence of resistance in a given area.

SUBMITTER: Dela Cruz WP

PROVIDER: S-EPMC1939917 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature

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Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs.

Dela Cruz Wilfred P WP Richardson Joann Y JY Broestler Judith M JM Thornton Jennifer A JA Danaher Patrick J PJ

Infectious diseases in obstetrics and gynecology 20070101

<h4>Objective</h4>Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS).<h4>Methods</h4>The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion.<h4>Results</h4>The presence of an erythromycin ribosome methylation gene (ermB or e ...[more]

PMID: 17710240

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Project description:Mycoplasma synoviae infection occurs worldwide, leading to considerable economic losses in the chicken and turkey industry due to infectious synovitis, respiratory diseases and eggshell apex abnormalities. Control programs against M. synoviae infection are based on eradication, vaccination and medication with antimicrobial agents. Prudent use of antibiotics can be improved greatly by the determination of antibiotic susceptibility prior to the treatment. However, the conventional broth or agar microdilution is very labor-intensive and time-consuming method. Thus, there is an increasing need for rapid antimicrobial susceptibility tests in order to guide antibiotic therapy more effectively. The aim of this study was to develop mismatch amplification mutation assays (MAMAs) to detect resistance-associated mutations in M. synoviae. M. synoviae strains with previously determined minimal inhibitory concentrations (MICs) and whole genomes (n = 92) were used for target selection and assay specification. For the evaluation of the developed assays, 20 clinical samples and an additional 20 M. synoviae isolates derived from these specimens were also included in this study. MIC values of these 20 isolates were determined by broth microdilution method. Five MAMAs were designed to identify elevated MICs of fluoroquinolones, while three MAMAs were developed to detect decreased susceptibility to macrolides and lincomycin. The sensitivity of the MAMA tests varied between 102-104 template copy number/reaction depending on the assay. Clinical samples showed identical genotype calls with the M. synoviae isolates derived from the corresponding specimens in each case. Supporting the results of conventional in vitro sensitivity tests, our approach provides a feasible tool for diagnostics. Rapidity, robustness and cost-effectiveness are powerful advantages of the developed assays. Supporting prudent antibiotic usage instead of empirical treatment, the use of this method can reduce significantly the economic impact of M. synoviae in the poultry industry and decrease bacterial resistance-related public health concerns.

Dataset Information

Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs.

Publications

Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs.

Similar Datasets

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