Quantification of fibronectin matrix assembly sites using a novel ELISA assay.
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ABSTRACT:
SUBMITTER: Zheng M
PROVIDER: S-EPMC1940232 | biostudies-literature | 2007 May
REPOSITORIES: biostudies-literature
Publications
Quantification of fibronectin matrix assembly sites using a novel ELISA assay.
Zheng Mingzhe M Ambesi Anthony A Yu Lin L McKeown-Longo Paula J PJ
Matrix biology : journal of the International Society for Matrix Biology 20061229 4
Binding of the N-terminus of fibronectin to assembly sites on the cell surface is an essential step in fibronectin fibrillogenesis. Fibronectin matrix assembly sites have customarily been quantified using an iodinated 70 kDa N-terminal fibronectin fragment. The 125I-70 K fragment is a less than ideal reagent because its preparation requires large amounts of plasma fibronectin and it has a fairly short shelf life. An additional limitation is that the cells responsible for binding the 125I-70 K ca ...[more]
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