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Identification of a new HLA-B allele (B*1576) by haplotype specific extraction.

ABSTRACT: Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is a convenient and simple tool for resolving ambiguous and novel allele combinations without the need for amplification or subcloning.

SUBMITTER: Dapprich J

PROVIDER: S-EPMC1945813 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Identification of a new HLA-B allele (B*1576) by haplotype specific extraction.

Dapprich Johannes J Witter Klaus K Gabel Harrison W HW Murphy Nancy B NB Albert Ekkehard D ED

Human immunology 20070215 5

Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is ...[more]

PMID: 17462508

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