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Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.

ABSTRACT: A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.

SUBMITTER: Gray M 

PROVIDER: S-EPMC194946 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature

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Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.

Gray M M   Wawrik B B   Paul J J   Casper E E  

Applied and environmental microbiology 20030901 9

A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment. ...[more]

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