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Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry.

ABSTRACT: The identification of post-translational modifications is difficult especially for hydrophobic membrane proteins. Here we present the identification of several types of protein modifications on membrane proteins isolated from mitochondrial outer membranes. We show, in vivo, that the mature rat liver mitochondrial carnitine palmitoyltransferase-I enzyme is N-terminally acetylated, phosphorylated on two threonine residues, and nitrated on two tyrosine residues. We show that long chain acyl-CoA synthetase 1 is acetylated at both the N-terminal end and at a lysine residue and tyrosine residues are found to be phosphorylated and nitrated. For the three voltage-dependent anion channel isoforms present in the mitochondria, the N-terminal regions of the protein were determined and sites of phosphorylation were identified. These novel findings raise questions about regulatory aspects of carnitine palmitoyltransferase-I, long chain acyl-CoA synthetase and voltage dependent anion channel and further studies should advance our understanding about regulation of mitochondrial fatty acid oxidation in general and these three proteins in specific.

SUBMITTER: Distler AM

PROVIDER: S-EPMC1950290 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry.

Distler Anne M AM Kerner Janos J Hoppel Charles L CL

Biochimica et biophysica acta 20070328 5

The identification of post-translational modifications is difficult especially for hydrophobic membrane proteins. Here we present the identification of several types of protein modifications on membrane proteins isolated from mitochondrial outer membranes. We show, in vivo, that the mature rat liver mitochondrial carnitine palmitoyltransferase-I enzyme is N-terminally acetylated, phosphorylated on two threonine residues, and nitrated on two tyrosine residues. We show that long chain acyl-CoA syn ...[more]

PMID: 17478130

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Dataset Information

Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry.

Publications

Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry.

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