Self-formed adaptor PCR: a simple and efficient method for chromosome walking.
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ABSTRACT:
SUBMITTER: Wang S
PROVIDER: S-EPMC1951051 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Publications
Self-formed adaptor PCR: a simple and efficient method for chromosome walking.
Wang Shiming S He Jian J Cui Zhongli Z Li Shunpeng S
Applied and environmental microbiology 20070504 15
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes. ...[more]
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