Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding.
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ABSTRACT:
SUBMITTER: Barjon C
PROVIDER: S-EPMC1951882 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Publications
Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding.
Barjon Clément C Wecker Karine K Izadi-Pruneyre Nadia N Delepelaire Philippe P
Journal of bacteriology 20070504 14
On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro. ...[more]
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