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Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.

ABSTRACT: Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.

SUBMITTER: Kachalova GS

PROVIDER: S-EPMC1952280 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.

Kachalova Galina S GS Rogulin Eugeny A EA Artyukh Rimma I RI Perevyazova Tatyana A TA Zheleznaya Ludmila A LA Matvienko Nickolay I NI Bartunik Hans D HD

Acta crystallographica. Section F, Structural biology and crystallization communications 20050301 Pt 3

Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume. ...[more]

PMID: 16511033

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