Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea).
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ABSTRACT:
SUBMITTER: Katre UV
PROVIDER: S-EPMC1952404 | biostudies-literature | 2005 Jan
REPOSITORIES: biostudies-literature
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Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea).
Katre Uma V UV Gaikwad S M SM Bhagyawant S S SS Deshpande U D UD Khan M I MI Suresh C G CG
Acta crystallographica. Section F, Structural biology and crystallization communications 20041224 Pt 1
The lectin isolated from mature seeds of Cicer arietinum (CAL) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. The purified lectin is a dimer of molecular weight 43,000 Da composed of two identical subunits (MW 21,500), as confirmed by SDS-PAGE. The lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 K over a well solutio ...[more]
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