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Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene.


ABSTRACT: ADAM33 has been identified as an asthma-associated gene in an out-bred population. Genetic studies suggested that the functional role of this metalloprotease was in airway remodeling. However, the mechanistic roles of the disease-associated SNPs have yet to be elucidated especially in the context of the pathophysiology of asthma. One disease-associated SNP, BC+1, which resides in intron BC toward the 5' end of ADAM33, is highly associated with the disease.The region surrounding this genetic variant was cloned into a model system to determine if there is a regulatory element within this intron that influences transcription.The BC+1 protective allele did not impose any affect on the transcription of the reporter gene. However, the at-risk allele enforced such a repressive affect on the promoter that no protein product from the reporter gene was detected. These results indicated that there exists within intron BC a regulatory element that acts as a repressor for gene expression. Moreover, since SNP BC+1 is a common genetic variant, this region may interact with other undefined regulatory elements within ADAM33 to provide a rheostat effect, which modulates pre-mRNA processing. Thus, SNP BC+1 may have an important role in the modulation of ADAM33 gene expression.These data provide for the first time a functional role for a disease-associated SNP in ADAM33 and begin to shed light on the deregulation of this gene in the pathophysiology of asthma.

SUBMITTER: Del Mastro RG 

PROVIDER: S-EPMC1955437 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature

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Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene.

Del Mastro Richard G RG   Turenne Laura L   Giese Heidi H   Keith Tim P TP   Van Eerdewegh Paul P   May Klaus J W KJ   Little Randall D RD  

BMC medical genetics 20070717


<h4>Background</h4>ADAM33 has been identified as an asthma-associated gene in an out-bred population. Genetic studies suggested that the functional role of this metalloprotease was in airway remodeling. However, the mechanistic roles of the disease-associated SNPs have yet to be elucidated especially in the context of the pathophysiology of asthma. One disease-associated SNP, BC+1, which resides in intron BC toward the 5' end of ADAM33, is highly associated with the disease.<h4>Methods</h4>The r  ...[more]

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