Ontology highlight
ABSTRACT:
SUBMITTER: Pestova TV
PROVIDER: S-EPMC195975 | biostudies-literature | 2003 Jan
REPOSITORIES: biostudies-literature
Pestova Tatyana V TV Hellen Christopher U T CU
Genes & development 20030101 2
Reconstitution of translation elongation from purified components confirmed that ribosomes that assembled on the Cricket paralysis virus intercistronic internal ribosomal entry site (IRES) without the involvement of initiation factors or initiator tRNA were active in elongation and are, therefore, true initiation complexes. The first elongation cycle occurred without peptide bond formation on 80S ribosomes that did not contain tRNA in the P site. It required elongation factors 1A and 2 and A sit ...[more]