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Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos.

ABSTRACT: Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.

SUBMITTER: Raya A

PROVIDER: S-EPMC196060 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos.

Raya Angel A Kawakami Yasuhiko Y Rodriguez-Esteban Concepcion C Buscher Dirk D Koth Christopher M CM Itoh Tohru T Morita Masanobu M Raya R Marina RM Dubova Ilir I Bessa Joaquin Grego JG de la Pompa Jose Luis JL Izpisua Belmonte Juan Carlos JC

Genes & development 20030502 10

Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship ...[more]

PMID: 12730123

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