Unknown

Dataset Information

0

P-cluster maturation on nitrogenase MoFe protein.


ABSTRACT: Biosynthesis of nitrogenase P-cluster has attracted considerable attention because it is biologically important and chemically unprecedented. Previous studies suggest that P-cluster is formed from a precursor consisting of paired [4Fe-4S]-like clusters and that P-cluster is assembled stepwise on MoFe protein, i.e., one cluster is assembled before the other. Here, we specifically tackle the assembly of the second P-cluster by combined biochemical and spectroscopic approaches. By using a P-cluster maturation assay that is based on purified components, we show that the maturation of the second P-cluster requires the concerted action of NifZ, Fe protein, and MgATP and that the action of NifZ is required before that of Fe protein/MgATP, suggesting that NifZ may act as a chaperone that facilitates the subsequent action of Fe protein/MgATP. Furthermore, we provide spectroscopic evidence that the [4Fe-4S] cluster-like fragments can be converted to P-clusters, thereby firmly establishing the physiological relevance of the previously identified P-cluster precursor.

SUBMITTER: Hu Y 

PROVIDER: S-EPMC1965529 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

P-cluster maturation on nitrogenase MoFe protein.

Hu Yilin Y   Fay Aaron W AW   Lee Chi Chung CC   Ribbe Markus W MW  

Proceedings of the National Academy of Sciences of the United States of America 20070611 25


Biosynthesis of nitrogenase P-cluster has attracted considerable attention because it is biologically important and chemically unprecedented. Previous studies suggest that P-cluster is formed from a precursor consisting of paired [4Fe-4S]-like clusters and that P-cluster is assembled stepwise on MoFe protein, i.e., one cluster is assembled before the other. Here, we specifically tackle the assembly of the second P-cluster by combined biochemical and spectroscopic approaches. By using a P-cluster  ...[more]

Similar Datasets

| S-EPMC8991001 | biostudies-literature
| S-EPMC2819195 | biostudies-literature
| S-EPMC3891402 | biostudies-literature
| S-EPMC7317204 | biostudies-literature
| S-EPMC4321486 | biostudies-literature
| S-EPMC3932303 | biostudies-literature
| S-EPMC3970913 | biostudies-literature
| S-EPMC4510703 | biostudies-literature
| S-EPMC5460594 | biostudies-literature
| S-EPMC1218249 | biostudies-other