Ontology highlight
ABSTRACT:
SUBMITTER: Hu Y
PROVIDER: S-EPMC1965529 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
Hu Yilin Y Fay Aaron W AW Lee Chi Chung CC Ribbe Markus W MW
Proceedings of the National Academy of Sciences of the United States of America 20070611 25
Biosynthesis of nitrogenase P-cluster has attracted considerable attention because it is biologically important and chemically unprecedented. Previous studies suggest that P-cluster is formed from a precursor consisting of paired [4Fe-4S]-like clusters and that P-cluster is assembled stepwise on MoFe protein, i.e., one cluster is assembled before the other. Here, we specifically tackle the assembly of the second P-cluster by combined biochemical and spectroscopic approaches. By using a P-cluster ...[more]