Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis.

ABSTRACT:

Background

After anaphase, the segregated chromosomes are sequestered by cytokinesis into two separate daughter cells by a cleavage furrow formed by the actomyosin-based contractile ring. The failure to properly position the contractile ring between the segregated chromosomes can result in aneuploidy. In both C. elegans embryos and human cells, the central spindle regulates division-plane positioning in parallel with a second pathway that involves astral microtubules.

Results

We combined genetic and pharmacological manipulations with live cell imaging to spatially separate the two division cues in a single cell. We demonstrate that the two pathways for furrow formation are mechanistically and genetically distinct. By following the distribution of green fluorescent protein (G

SUBMITTER: Werner M 

PROVIDER: S-EPMC1978103 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature