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Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7.

ABSTRACT: ST0811 from Sulfolobus tokodaii strain 7, a member of the YjgF/YER057c/UK114 protein family, was crystallized by the sitting-drop vapour-diffusion method using PEG 10,000 as precipitant. The crystals diffracted X-rays to beyond 2.0 A resolution using an in-house rotating-anode generator. The crystals belonged to the rhombohedral space group R3, with hexagonal unit-cell parameters a = b = 55.0, c = 223.2 A. The crystals contained two molecules in the asymmetric unit (VM = 2.3 A3 Da(-1)) and had a solvent content of 47%.

SUBMITTER: Miyakawa T

PROVIDER: S-EPMC1978107 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7.

Miyakawa Takuya T Hatano Ken Ichi KI Lee Woo Cheol WC Kato Yusuke Y Sawano Yoriko Y Yumoto Fumiaki F Nagata Koji K Tanokura Masaru M

Acta crystallographica. Section F, Structural biology and crystallization communications 20050831 Pt 9

ST0811 from Sulfolobus tokodaii strain 7, a member of the YjgF/YER057c/UK114 protein family, was crystallized by the sitting-drop vapour-diffusion method using PEG 10,000 as precipitant. The crystals diffracted X-rays to beyond 2.0 A resolution using an in-house rotating-anode generator. The crystals belonged to the rhombohedral space group R3, with hexagonal unit-cell parameters a = b = 55.0, c = 223.2 A. The crystals contained two molecules in the asymmetric unit (VM = 2.3 A3 Da(-1)) and had a ...[more]

PMID: 16511170

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