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ABSTRACT:
SUBMITTER: Iwaki J
PROVIDER: S-EPMC1978129 | biostudies-literature | 2005 Nov
REPOSITORIES: biostudies-literature
Iwaki Jun J Suzuki Ryuichiro R Fujimoto Zui Z Momma Mitsuru M Kuno Atsushi A Hasegawa Tsunemi T
Acta crystallographica. Section F, Structural biology and crystallization communications 20051025 Pt 11
Hyperthermophilic archaeal tyrosyl-tRNA synthetase from Aeropyrum pernix K1 was cloned and overexpressed in Escherichia coli. The expressed protein was purified by Cibacron Blue affinity chromatography following heat treatment at 363 K. Crystals suitable for X-ray diffraction studies were obtained under optimized crystallization conditions in the presence of 1.5 M ammonium sulfate using the hanging-drop vapour-diffusion method. The crystals belonged to the tetragonal space group P4(3)2(1)2, with ...[more]