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Human RFP2 gene promoter: unique structure and unusual strength.

ABSTRACT: Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5'-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5'-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.

SUBMITTER: Skoblov M

PROVIDER: S-EPMC1994241 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature

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Human RFP2 gene promoter: unique structure and unusual strength.

Skoblov Mikhail M Shakhbazov Konstantin K Oshchepkov Dmitry D Ivanov Dmitry D Guskova Anna A Ivanov Dmitry D Rubtsov Petr P Prasolov Vladimir V Yankovsky Nick N Baranova Ancha A

Biochemical and biophysical research communications 20060217 3

Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5'-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5'-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined S ...[more]

PMID: 16499869

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