Ontology highlight
ABSTRACT:
SUBMITTER: Gu J
PROVIDER: S-EPMC2034460 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Gu Jiafeng J Lu Haihui H Tsai Albert G AG Schwarz Klaus K Lieber Michael R MR
Nucleic acids research 20070823 17
The double-strand DNA break repair pathway, non-homologous DNA end joining (NHEJ), is distinctive for the flexibility of its nuclease, polymerase and ligase activities. Here we find that the joining of ends by XRCC4-ligase IV is markedly influenced by the terminal sequence, and a steric hindrance model can account for this. XLF (Cernunnos) stimulates the joining of both incompatible DNA ends and compatible DNA ends at physiologic concentrations of Mg2+, but only of incompatible DNA ends at highe ...[more]