A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons.
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ABSTRACT:
SUBMITTER: Chateigner-Boutin AL
PROVIDER: S-EPMC2034463 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Publications
A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons.
Chateigner-Boutin Anne-Laure AL Small Ian I
Nucleic acids research 20070828 17
We describe a rapid, high-throughput method to scan for new RNA editing sites. This method is adapted from high-resolution melting (HRM) analysis of amplicons, a technique used in clinical research to detect mutations in genomes. The assay was validated by the discovery of six new editing sites in different chloroplast transcripts of Arabidopsis thaliana. A screen of a collection of mutants uncovered a mutant defective for editing of one of the newly discovered sites. We successfully adapted the ...[more]
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