Ontology highlight
ABSTRACT:
SUBMITTER: Kavran JM
PROVIDER: S-EPMC2040888 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Kavran Jennifer M JM Gundllapalli Sarath S O'Donoghue Patrick P Englert Markus M Söll Dieter D Steitz Thomas A TA
Proceedings of the National Academy of Sciences of the United States of America 20070625 27
Pyrrolysine (Pyl), the 22nd natural amino acid and genetically encoded by UAG, becomes attached to its cognate tRNA by pyrrolysyl-tRNA synthetase (PylRS). We have determined three crystal structures of the Methanosarcina mazei PylRS complexed with either AMP-PNP, Pyl-AMP plus pyrophosphate, or the Pyl analogue N-epsilon-[(cylopentyloxy)carbonyl]-L-lysine plus ATP. The structures reveal that PylRS utilizes a deep hydrophobic pocket for recognition of the Pyl side chain. A comparison of these stru ...[more]