Ontology highlight
ABSTRACT:
SUBMITTER: Godornes C
PROVIDER: S-EPMC2041851 | biostudies-literature | 2007 Apr
REPOSITORIES: biostudies-literature
Godornes Charmie C Leader Brandon Troy BT Molini Barbara J BJ Centurion-Lara Arturo A Lukehart Sheila A SA
Cytokine 20070401 1
Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues. We report the development of a quantitative real-time RT-PCR method for measuring the relative levels of rabbit IFN-gamma, IL-2, IL-4, IL-10 and TNF-alpha mRNA. Quantitation was accomplished by comparison to ...[more]