Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses.
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ABSTRACT:
SUBMITTER: Coute Y
PROVIDER: S-EPMC2042066 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses.
Couté Yohann Y Hernandez Céline C Appel Ron D RD Sanchez Jean-Charles JC Margolles Abelardo A
Applied and environmental microbiology 20070629 17
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence. ...[more]
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